DETERMINATION OF THE TRANSMISSION OF Ralstonia solanacearum
IN TRUE POTATO SEED (TPS) AND TOMATO SEED.
SAENZ, E. C.; BORDA, C. C.; GUTARRA, L. & PRIOU,
S. Determinação da transmissão
de Ralstonia solanacearum na semente verdadeira de batata
(TPS) e semente de tomate.
Bacterial wild caused by the soilborne bacterium R.
solanacearum, is one of the most destructive bacterial
diseases of economically important crops. Tomato seed
and mesocarp extracts were inoculated with different
concentrations of R. solanacearum race 3/biovar II (10
to 108 ufc/ml in tenfold serial dilutions) and incubated
at room temperature for 6, 12, 24 and 48 hours with
constant agitation (150 r.p.m.) previously incubated
at 40C for 2 hours. After enrichment, the extracts were
analyzed in plated on medium SMSA, ELISA and double-PCR
to repeat the methodology with TPS and potato mesocarp.The
method developed in tomato seed inoculated was applied
to samples of tomato seed and mesocarp obtained from
plants with bacterial wild symptoms from field infested
and plants inoculated in greenhouse with R. solanacearum,
were analyzed to check the effectiveness of the detection
method. Different infected plant parts (roots, stem
and fruit) were examined to confirm the presence of
the pathogen. The analyses demonstrated the method affectiviness
and that R. solanacearum is a bacterial that infects
tomato seed. Finally, it was examined samples of TPS
and mesocarp of two resistant varieties and two susceptible
varieties of potato, obtained from plants with bacterial
wild symptoms from fields infected. The analyzes demonstrated
that neither TPS nor mesocarp from wild plants of 4
varieties harbor R. solanacearum.